Rooted in Classics, A New Chapter for Cell Counting - BodBoge

Current： Home > News

Rooted in Classics, A New Chapter for Cell Counting

Release time：2024.02.26 Views：816

Cell concentration calculation is routine in cell experiments to set seeding density. Automated cell counters offer rapid detection, yet studies reveal inconsistent accuracy for complex cell samples due to divergent cell morphology recognition algorithms.

In clinical testing, automated hematology analyzers often fail to accurately distinguish cell morphology, requiring calibration with the standard Neubauer hemocytometer. Manual microscopic counting with hemocytometers is a universally accepted reference method.

Specifications & Counting Principle of Hemocytometer

A classic counting tool with two identical counting chambers.Cover glass placement creates a 0.10 mm deep chamber. Each chamber contains 9 large squares of 1.00 mm × 1.00 mm.Limited microscopic vision leads to high counting errors, so standard practice counts the four corner large squares. For tiny cells like red blood cells, 5 sub-zones within the central large square are counted instead.

Concentration formula:Cell Density (cells/mL) = (Total cells in 4 corner squares ÷ 4) × Dilution Factor × 10⁴

Counting Principle of Bodboge Cell Counters

Bodboge cell counters are developed based on the standard hemocytometer counting principle, complying with JJG 552-1988 Verification Regulation for Hemocytometers. Compatible disposable counting slides share the identical chamber depth as standard hemocytometers.

①When paired with a hemocytometer, the instrument acts as an automated microscopic imaging workstation with integrated computer. It delivers real-time HD cell preview, precise 3-axis motorized positioning and automatic focusing for sharp images, with built-in algorithms for automatic cell identification and counting.

②For disposable counting slides, virtual graticules are displayed on screen. Calibrated via the simulated hemocytometer utility model patent (ZL 202021855359.4), each grid matches the 1×1 mm² area of traditional hemocytometers.

③Disposable slides feature a precisely calibrated 0.1 mm chamber depth, yielding the same 0.1 mm³ counting volume as hemocytometers.

④The identical standard concentration formula applies to both consumables, enabling direct manual cross-verification of counting results.

Note: Certain models omit hemocytometer compatibility, while retaining the same core algorithm design. Contact Botuboju Technology Co., Ltd. for model-specific details.

Innovative Counting System by Bodboge

instruments support multiple counting modes: unstained counting (Patent ZL 201310101403.0), trypan blue staining and fluorescent staining.Preloaded recognition templates cover common cell types. Customizable parameters are available for complex, atypical samples, with unlimited template creation.

Combining traditional reference standards and innovative automated technology, Botuboju delivers comprehensive solutions for cell counting and analysis.

References：

[1] .Borzova E. The Absolute Basophil Count. Methods Mol Biol. 2020;2163:109-124.

[2] Zhang M, Gu L, Zheng P, et al. Improvement of cell counting method for Neubauer counting chamber. J Clin Lab Anal. 2020;34:e23024.