Preface & Background

The 2008 Nobel Prize in Chemistry was awarded to Osamu Shimomura, Martin Chalfie and Roger Y. Tsien in recognition of their landmark discovery and research on Green Fluorescent Protein (GFP).

Transfection is a routine molecular biology technique that introduces exogenous genes into cells to explore the expression and biological functions of target genes.

Fluorescence Microscopic Observation

After fusing the GFP gene with target genes and delivering the constructs into cells, researchers can track intracellular target gene expression via GFP fluorescence signals. This process traditionally relies on fluorescence microscopy to count GFP-positive cells.

(Cell Transfection Flowchart)

Nevertheless, manual microscopic counting has prominent drawbacks: cumbersome workflows, heavy time and labor costs, and significant subjective bias from operators, leading to unstable counting accuracy.

Automated fluorescent cell analyzers effectively solve these pain points.

Automated Fluorescent Cell Counter & Analyzer

Botuboju fluorescent cell counters and analyzers are equipped with a dedicated transfection assay mode, optimized for cells labeled with GFP, RFP and other customizable fluorescent proteins. Built-in intelligent algorithms enable automatic counting and quantitative analysis of fluorescent cells to eliminate human error.

High-definition cell images and comprehensive statistical data are displayed intuitively, facilitating result interpretation and academic reporting.

Instrument Imaging & Analysis Report

The analytical report covers: bright-field cell count & concentration, transfected cell count & concentration, transfection efficiency, original cell images and labeled segmentation images.

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